Journal: Molecular Brain
Article Title: Cacna1b alternative splicing impacts excitatory neurotransmission and is linked to behavioral responses to aversive stimuli
doi: 10.1186/s13041-019-0500-1
Figure Lengend Snippet: Replacing e37a- Cacna1b mRNA by e37b-Cacna1b results in enhanced probability of transmitter release in mPP-DG synapses. a Schematic describing approximate placement of recording (rec) and stimulation (stim) electrodes in the superior blade of DG and s.r. in CA1. b I/O relationships of fEPSPs from mPP-DG synapses recorded in WT and e37b-only mice. Slopes of fEPSPs were plotted as a function of the stimulus intensity. c I/O of fEPSPs from SC-CA1 synapses recorded in WT and e37b-only mice. Slopes of fEPSPs were plotted as a function of the stimulus intensity. d Representative traces of mPP-DG fEPSPs using a PPR protocol in WT and e37b-only slices ( left panel ). PPR of fEPSPs recorded at varying pulse intervals in slices of WT and e37b-only ( right panel ). e Representative traces of SC-CA1 fEPSPs using a PPR protocol in WT and e37b-only slices ( left panel ). PPR of fEPSPs recorded at varying pulse intervals in slices of WT and e37b-only mice ( right panel ). b-e . Data are shown as mean ± SE
Article Snippet: A bipolar tungsten stimulation electrode (FHC Inc) was placed in nearby in this layer of DG and the medial perforant path (mPP) was identified by pre-pulse inhibition.
Techniques: